DeepSight 3D Lightsheet Microscope

DeepSight 3D Lightsheet Microscope 

Multi-scale  |  Larger Tissues   |  Higher Resolution   |  Faster Imaging 3D Imaging   |  In Vivo Compatible

‍

A High-Resolution Lightsheet Platform for Multi-scale Clearing Samples, Multi-Color 3D Imaging, and In vivo Observation

The DeepSight 3D Lightsheet Microscope series allows for real-time light sheet adjustment and automatic calibration, ensuring ease of use and stability across diverse applications. Furthermore, tailored to the characteristics of different samples, the DeepSight 3D series offers optimized accesso-ries and workflow protocols, enabling users to adjust imaging resolution, speed, magnification, and sample processing methods. This facilitates the acquisition of multi-color structural information at micron, sub-micron, or even hundred-nanometer - level 3D spatial resolution. The accompanying image processing and analysis platform empowers users to efficiently analyze and interpret results. This makes the system widely applicable in funda-mental life science research areas such as neurosci-ence, oncology, and developmental biology, as well as in pharmaceutical fields including drug develop-ment, pharmacodynamic evaluation, and clinical pathology.

Multi-scale Imaging from Micro to Macro

Our superior optical design, paired with multiple sample chamber formats, enables flexible lightsheet focusing across distanc-es of several centimeters—delivering precise and sharp images whether you're imaging 100-μm organoids, millimeter-thick tissue slices, or whole mice at the centimeter scale. The ultra-long travel range works in tandem with high-precision stepping to provide ample imaging depth while retaining nanometer-level positioning. Combined with long-working-distance, high-NA objectives, our system maintains a generous field of view without sacrificing resolution. It supports full-body scanning of an adult mouse, yet can instantly switch to high magnification to reveal fine internal structures in minute samples like organoids. The result is a truly versatile platform that makes cross-scale research simple, seamless, and efficient.

One-Stop 3D Imaging Service for All Types of Cleared Samples

Imaging strategies for cleared tissues often depend on tissue type, fluorescent labeling methods, sample size, and the clearing system used. The DeepSight 3D Lightsheet Microscope series is designed for such complex samples and is compatible with hydrophilic, hydrophobic, and hydrogel-based clearing methods. 

For cleared samples like mouse brains, hearts, livers, spleens, lungs, kidneys, embryos, and spinal cords, the value of complete 3D imaging lies in preserving structural continuity. Researchers are no longer reliant on local sections or stitching multiple dissected pieces; they can observe overall morphology, spatial distribution, and local details within a single, intact dataset.

Proprietary Tiling Technology Improving Quality and Stability

The DeepSight 3D Lightsheet Microscope series can be equipped with Nuohai's proprietary Tiling (Virtual Scan-ning) Light Sheet Technology, which allows for light sheet control and optimization across a large field of view. Compared to a single light sheet covering the entire field of view, the tiling technology can generate multiple light sheets successively along the light propagation direction, maintaining light sheet thickness, illumination uniformity, and effective optical sectioning over larger areas, thereby alleviating the inherent conflict between field of view, resolution, and signal-to-noise ratio.

Automatic Beam Calibration for Superior Usability and Reliability

The light sheet may shift laterally or along the Z-axis when imaging media, excitation wavelengths, or magnification levels change. The DeepSight 3D Lightsheet Microscope series offers built-in calibration routines that help preserve spatial consistency across multicolor acquisitions and different clearing protocols—minimizing the uncertainty of manual re-adjustments. A fast preview mode enables rapid sample navigation and ROI localization, while high-resolu-tion mode captures fine structural details for validation. Together, they ensure a smooth workflow from rapid screening to high-quality data collection.

Low-Phototoxicity, High-Speed Imaging for Long-Term In Vivo Imaging

By separating illumination from detection, our system excites only the fluorescence signal at the current imaging plane. Coupled with an area-array sCMOS camera, it captures 3D images with minimal out-of-focus excitation—substantially reducing photobleaching and phototoxicity. When paired with our in vivo imaging module, the system is ideally suited for long-term, time-lapse observation of sensitive specimens such as zebrafish and organoids.

A Complete Workflow for Large Datasets

For users, the DeepSight 3D Lightsheet Microscope series is more than just a microscope; it is an entry point to a complete imaging workflow, from acquisition control and real-time preview to data pre-processing and automatic stitching. After the system control software completes data acquisition from a specified region, users can flexibly call upon the accompanying image processing and analysis platform for subsequent processing, visualization, and analy-sis. This helps users convert large-sample image data into observable, comparable, and analyzable 3D results.