锘海生物提供组织透明化3D平铺光片显微镜成像及数据分析与处理整体解决方案

DeepSight 3D Lightsheet Microscope Combined with Tissue Clearing: Unlock the "Golden Connectome" of the Central Nervous System!

Beyond local observation: Using a Thy1-GFP transgenic mouse (gold-labeled neurons) with Nuohai Hydrophilic/Enhanced Tissue Clearing Kit and DeepSight 3D Lightsheet Microscope, to realize full 3D visualization of neuronal signals across the whole central nervous system. Our clearing workflow delivers high processing efficiency with outstanding endogenous fluorescence retention and good compatibility with immunostaining. Notably, whole adult mouse brain imaging can be completed in as little as 10 seconds! From neuronal connectivity and functional distribution to pathological aberrations in disease models, comprehensive whole-brain 3D structural information can be acquired with one single operation.

Mouse Thick Brain Section – 4× Expansion: Decoding the "High-Definition Code" of Dendritic Spines

Restricted by the diffraction limit, conventional slice imaging always leaves fine structures like dendritic spines blurred and indistinct. Using 4× physical expansion combined with DeepSight 3D Lightsheet Microscope, we push the resolution of Thy1-GFP-labeled neurons (gold) to the hundred-nanometer level – the morphology, density, and spatial distribution of dendritic spines are revealed in thick brain sections with unprecedented clarity! The seamless integration of the expansion kit with light sheet imaging renders axonal trajectories and synaptic connections in exquisite detail, building a brand-new high-resolution platform for researches on neural circuit plasticity.

3D Brain Atlas & Whole-Brain c-Fos Quantification: Acquire Comprehensive Neural Activity Mapping in One Workflow

Confused about activated brain regions under specific stimuli? Nuohai provides an all-in-one solution covering whole-brain c-Fos labeling and quantitative analysis. Our Tissue Clearing Kit is compatible with immunofluorescence staining while well preserving transgenic c-Fos signals. Combined with high-speed DeepSight 3D Lightsheet Microscopy, the system displays the spatial distribution of c-Fos positive cells from global to local scales. Furthermore, the platform enables targeted brain region tracking and accurate quantification of cell number, cellular density and fluorescence intensity. Whether for research on learning & memory, emotional modulation, pain perception or drug functional mechanisms, quantitative neural activity maps can be generated instantly to intuitively reflect brain responses against behavioral stimuli.