3D Panoramic Profiling: Multicolor Fluorescence Reconstruct the Microscopic Landscape of Organoids

YOYO (Cyan) dye stains cell nuclei to reflect overall cellular density and spatial layout inside organoids; α-SMA (Yellow) marks smooth muscle cells and myofibroblasts, commonly used to observe vascular walls, stromal contractile structures, or tissue mechanical support networks; Lectin (Magenta) binds specifically to glycoproteins to label endothelial cells and vascular networks, acting as a core biomarker for evaluating organoid vascularization efficiency. Superposition of the three fluorescence channels not only displays cellular heterogeneity inside organoids, but also reflects the functional maturation and structural complexity of engineered tissues. This technology provides critical intuitive evidence for disease model construction and preclinical drug screening!

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膨胀64倍后组织透明化三维成像

St.23海鞘胚胎活体成像 

标记：细胞膜，成像分辨率：0.4×0.4×0.7um