3D Panoramic Imaging of Lymph Nodes: Fully Expose Tiny Metastatic Lesions

Traditional pathological diagnosis relying on 2D tissue slices frequently misses micro metastatic foci. We employ CK19 (green channel) to label epithelial metastatic cells and Ki67 (red channel) to highlight proliferative active regions. Supported by high-resolution 3D imaging via the DeepSight 3D Lightsheet Microscope, proliferative and highly invasive core metastatic lesions within lymph nodes can be precisely localized, with lesion morphology and spatial distribution fully characterized alongside improved detection sensitivity. This visualization tool facilitates researches on tumor metastatic mechanisms, personalized therapeutic formulation and precise prognostic evaluation.

Explore the Regulatory Hub of Pancreatic Islets: Dual GCG (Green) / TH (Red) Labeling Decodes Neuro-Endocrine Crosstalk!

Dual staining targeting glucagon (GCG) and tyrosine hydroxylase (TH) maps the spatial adjacency between pancreatic α-cells and sympathetic nerve fibers within human pancreatic tissues. This dual immunofluorescence strategy aims to reveal the innervation patterns of the pancreas, particularly the direct regulatory mechanisms of the sympathetic nervous system on α-cell secretory function. For instance, this method can verify whether sympathetic excitation boosts glucagon secretion via synaptic-like tight cell contacts under stress conditions, supplying solid morphological evidence for illustrating how neuroendocrine crosstalk modulates blood glucose homeostasis.

Non-Destructive 3D Pathology for Lung Tissues: Eliminate Observation Blind Zones from 2D Sectioning

Conventional lung slicing damages original samples and only delivers planar information, failing to reconstruct authentic in-situ spatial structures. Our scheme combines nuclear labeling (purple) with intrinsic tissue autofluorescence (pink) to achieve intact non-destructive 3D imaging. No slicing or tissue destruction is needed to reconstruct accurate 3D topological relations among alveoli, bronchi and pulmonary interstitium and eliminate section-induced blind areas. Quantifiable and objective imaging data provides more realistic and comprehensive pathological evidence for respiratory disease diagnosis.

舌癌活检样本

肠镜活检样本-ki67细胞增值指标