Product Description：

The kit provides a hydrophilic clearing method that achieves tissue clearing fast and efficiently for centimeter-sized tissue samples. The kit mainly increases the fluidity of the cell membrane through hydration, and combines the permeability of the detergent to the cell membrane to achieve the purpose of removing the lipids. Then, the samples are clearing by RI matching. This method is suitable for the tissue clearing of young embryo (The embryo is no older than 15 days) and organoid. The

samples after tissue clearing 

Product Features:

• Fast and gentle transparent immature biological tissue 

• Compatible with immunofluorescence staining and endogenous fluorescent protein labeling 

• High biosecurity 
  

• Suitable for common fluorescence microscopes

  
  

  
  

E14.5 mouse embryo was cleared with embryo clearing kit, labeled with anti-GFAP-561 (green) and licectin-AF647 (purple), and whole-body imaging was performed with LS18 tile light microscope. 561nm and 637nm lasers were used as excitation light sources, 0.25NA objective and 6.3× zoom were used to detect fluorescence signals to achieve 3D visualization of GFAP and lectin positive cells.

E14.5 mouse embryo was cleared with embryo clearing kit, nucleus was labeled with PI, and whole embryo image was performed with LS18 tile light microscope. 561nm laser was used as excitation light source, 0.25NA objective lens and 6.3× zoom were used to detect fluorescence signals to realize three-dimensional visualization of all mouse embryo nuclei.

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CAT#: NH-CR-230601