Understanding complex biological systems requires the system-wide characterization of both molecular and cellular features. Existing methods for spatial mapping of biomolecules in intact tissues suffer from information loss caused by degradation and tissue damage. We report a tissue transformation strategy named stabilization under harsh conditions via intramolecular epoxide linkages to prevent degradation (SHIELD), which uses a flexible polyepoxide to form controlled intra- and intermolecular cross-link with biomolecules. SHIELD preserves protein fluorescence and antigenicity, transcripts and tissue architecture under a wide range of harsh conditions. We applied SHIELD to interrogate system-level wiring, synaptic architecture, and molecular features of virally labeled neurons and their targets in mouse at single-cell resolution. We also demonstrated rapid three-dimensional phenotyping of core needle biopsies and human brain cells. SHIELD enables rapid, multiscale, integrated molecular phenotyping of both animal and clinical tissues.

About Nuohai Life Science

Nuohai Life Science co., Limited is committed to equipping scientists in life science field with advanced experimental and research & manufactural equipments, related consumables, reagents as well as offering professional application and technical support services. We aim to constantly promote the development of new technology in life science field with the timely introduction of the latest foreign technology and products. Meanwhile, Nuohai Life Science provide CRO and CMO service to acdamic and industrial customers, to satisfy every demand in the R&D and manufacturing. 

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