Rabbit spinal cord
Treated with Nuohaienhanced clearing reagent kit; 3D imaging by Nuohai LS18 tiling light sheetmicroscope;
Sample size:1.5cm*7cm*3cm
C57 full mouse
Treated with Nuohaitissue clearing reagent kit; 3D imaging by Nuohai LS18 tiling light sheetmicroscope; clear presentation of full mouse tissue distribution and shape.
Sample size:37.3mm*77.4mm*23.1mm
Mousehead details
Mouseupper limb details
Mousebreast details
Thy1-GFP Adult mouse –completed central nervous system
Treated with Nuohaitissue clearing reagent kit;
Resolution: 2μm×2μm×5μm
Thy1-eGFP mouse completed skull brain sample
Treated with Nuohaitissue clearing reagent kit; 3D imaging by Nuohai LS18 tiling light sheetmicroscope
Thy1-GFP positive neuron
Treated withNuohai tissue expansion reagent kit
0.25NAObjective
Resolution: 0.5μm×0.5μm×1.25μm
Thy1-GFP positive neuron
Treated with Nuohaitissue expansion reagent kit
0.95NAObjective
Resolution: 85nm×85nm×200nm
Mouse full brain with various fluorescent labelling
Treated with Nuohai hydrophilictissue clearing reagent kit and Nuohai enhanced tissue clearing reagent kit
SM22: large vasculature Lection: microvasculature TH: Dopaminergic neuron Systemto&PI:nucleus
0.25NAobjective
Resolution::4μm×4μm×10μm
BrainC-Fos Signal extraction
Treated with Nuohaienhanced clearing reagent kit
0.25NAobjective
Resolution:3.3μm×3.3μm×7μm
According to the Allen Brain Atlas, 14 distinctbrain regions were delineated and rendered in different colors
Cardiac vessel
Treated with Nuohaienhanced clearing reagent kit
Lectin:Lectin:Vasculature
0.25NAobjective
Resolution:2μm×2μm×5μm
Mouse lung
Treated with Nuohaitissue clearing reagent kit; 3D imaging byNuohai LS18 tiling light sheet microscope; clear 3D presentation of the vessel system.
Mouse Intestine
Treated with Nuohaienhanced clearing reagent kit
Lectin: ChAT-GFP
Lectin: Vasculature ChAT-GFP:Positive neuron
0.25NAobjective
Resolution:2μm×2μm×5μm
Human intestinalbiopsy
Ki67: Proliferating cell Cam5.2: Epithelial cell
0.6NAImmersion Objective
Resolution:0.52μm×0.52μm×1.4μm
Mouse Ovary
Treated with Nuohaienhanced clearing reagent kit
Oocytes:blue
0.6NA ImmersionObjective
Resolution:0.52μm×0.52μm×1μm
Ovarian Cancer Tissue
Ki67: Proliferating cell Cam5.2: Epithelial cell
0.25NAobjective
Resolution:2μm×2μm×5μm
MammaryTissue
Ki67: Proliferating cell Cam5.2: Epithelial cell
0.25NAobjective
Resolution:2μm×2μm×5μm
E12.5Mouse Embryo
Treatedwith Nuohai hydrophilic tissue clearing reagent kit
Sox10:Green Sox9:Red
0.25NAobjective
Resolution:2μm×2μm×5μm
ThyroidCancer Tissue
Ki67: Proliferating cell Cam5.2: Epithelial cell
0.25NAobjective
Resolution:2μm×2μm×5μm
Hela cell
Treatedwith Nuohai cell expansion reagent kit
Mito:Mitochondrion
Imaging by Confocal Microscope
Resolution:0.414μm×0.414μm×0.8μm
U-2 OSCell
Treatedwith Nuohai cell expansion reagent kit
Tubulin:Tubulin
Imaging by Confocal Microscope
Resolution:0.414μm×0.414μm×1μm
Colorectal LymphNode
CK19:Epithelialcell Lca: leukocyte surface antigen
0.25NAobjective
Resolution:3.3μm×3.3μm×7 μm
Colorectalpolyps
Ki67: Proliferating cell Cam5.2: Epithelial cell
Lca: leukocyte surface antigen
0.25NAobjective
Resolution:3.3μm×3.3μm×7μm
Mouse brain-glioma tumor and nano material
Glioma(red), nano material(green)
Tyrosine Hydroxylase staining reveals sympathetic nerve in an intact mouse heart
Cleared and labeled with our pipeline, imaged with LS18 at 3.2x.
小鼠心脏血管成像
Endogenous Thy1-driven EGFP expression visualized throughout a P15 mouse entire CNS (whole brain and spinal cord).
Cleared with our pipeline, imaged with LS18 at 4x
Thy1-GFP 阳性神经元
PI (Propidium Iodide) staining of an entire mouse brain hemisphere.
Cleared and labeled with our pipeline, imaged with LS18 at 3.2x.
Endogenous Tie2- tdTomato visualized throughout an entire adult mouse brain.
Cleared with our pipeline, imaged with LS18 at 4x.
Endogenous Thy1-driven EGFP expression visualized throughout an entire adult mouse brain.
Cleared with our pipeline, imaged with LS18 at 3.2x
Neurons of a brain sample in transgenic Thy1- EGFP mice.
Cleared with our pipeline, imaged with LS18 at 6.3x.
Tyrosine Hydroxylase staining reveals sympathetic nerve in a mouse lung
Cleared and labeled with our pipeline, imaged with LS18 at 3.2X.
小鼠肝脏神经
Endogenous Tie2- tdTomato visualized in a mouse liver.
Cleared with our pipeline, imaged with LS18 at 3.2x.
Endogenous Tie2- tdTomato visualized in a mouse stomach.
Cleared with our pipeline, imaged with LS18 at 3.2x.
Endogenous Tie2- tdTomato visualized in a mouse kidney.
Cleared with our pipeline, imaged with LS18 at 3.2X.
Tyrosine Hydroxylase staining reveals sympathetic nerve in an intact mouse spleen
Cleared and labeled with our pipeline, imaged with LS18 at 6.3x.
Endogenous Tie2- tdTomato visualized in a mouse testis.
Cleared with our pipeline, imaged with LS18 at 3.2x.
胎鼠卵巢
卵泡(红色)、细胞核(蓝色)
小鼠胚胎膨胀样品(细胞核&Lectin阳性细胞)
采用锘海组织膨胀试剂盒,LS18平铺光片显微镜6.3X倍率成像
小鼠胚胎神经
Mouse spleen labeled with Tyrosine Hydroxylase
Cleared and labeled with our pipeline, imaged with LS18 at 23x
小鼠脊髓神经元
小鼠肠血管及细节图
小鼠骨股双通道(图左) 间充质干细胞(图中) 基质细胞(图右)
斑马鱼