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One-Step workflow from tissue clearing, immunolabeling, 3D imaging to data analysis and storage.

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Rabbit spinal cord

Treated with Nuohaienhanced clearing reagent kit; 3D imaging by Nuohai LS18 tiling light sheetmicroscope;

Sample size:1.5cm*7cm*3cm


C57 full mouse

Treated with Nuohaitissue clearing reagent kit; 3D imaging by Nuohai LS18 tiling light sheetmicroscope; clear presentation of full mouse tissue distribution and shape.

Sample size:37.3mm*77.4mm*23.1mm


Mousehead details

Mouseupper limb details

Mousebreast details

Thy1-GFP Adult mouse –completed central nervous system

Treated with Nuohaitissue clearing reagent kit;  

Resolution: 2μm×2μm×5μm


Thy1-eGFP mouse completed skull brain sample

Treated with Nuohaitissue clearing reagent kit; 3D imaging by Nuohai LS18 tiling light sheetmicroscope


Thy1-GFP positive neuron

Treated withNuohai tissue expansion reagent kit

 0.25NAObjective

Resolution: 0.5μm×0.5μm×1.25μm




Thy1-GFP positive neuron

Treated with Nuohaitissue expansion reagent kit


  0.95NAObjective

Resolution: 85nm×85nm×200nm


Mouse full brain with various fluorescent labelling


Treated with Nuohai hydrophilictissue clearing reagent kit and Nuohai enhanced tissue clearing reagent kit


SM22: large vasculature  Lection: microvasculature  TH: Dopaminergic neuron Systemto&PI:nucleus

 0.25NAobjective

Resolution::4μm×4μm×10μm 



BrainC-Fos Signal extraction

Treated with Nuohaienhanced clearing reagent kit

 0.25NAobjective

Resolution:3.3μm×3.3μm×7μm

According to the Allen Brain Atlas, 14 distinctbrain regions were delineated and rendered in different colors


Cardiac vessel

Treated with Nuohaienhanced clearing reagent kit

Lectin:Lectin:Vasculature

 0.25NAobjective

Resolution:2μm×2μm×5μm


Mouse lung

Treated with Nuohaitissue clearing reagent kit; 3D imaging byNuohai LS18 tiling light sheet microscope; clear 3D presentation of the vessel system.

 

Mouse Intestine

Treated with Nuohaienhanced clearing reagent kit

Lectin: ChAT-GFP

Lectin: Vasculature ChAT-GFP:Positive neuron

 0.25NAobjective

Resolution:2μm×2μm×5μm   


Human intestinalbiopsy

Ki67: Proliferating cell   Cam5.2: Epithelial cell

 0.6NAImmersion Objective

  Resolution:0.52μm×0.52μm×1.4μm 


Mouse Ovary

Treated with Nuohaienhanced clearing reagent kit

Oocytes:blue

0.6NA ImmersionObjective

Resolution:0.52μm×0.52μm×1μm   


Ovarian Cancer Tissue

Ki67: Proliferating cell   Cam5.2: Epithelial cell

0.25NAobjective

Resolution:2μm×2μm×5μm   


MammaryTissue

Ki67: Proliferating cell   Cam5.2: Epithelial cell

  0.25NAobjective

Resolution:2μm×2μm×5μm  


E12.5Mouse Embryo

 Treatedwith Nuohai hydrophilic tissue clearing reagent kit

 Sox10:Green  Sox9:Red

0.25NAobjective

Resolution:2μm×2μm×5μm   


 ThyroidCancer Tissue

Ki67: Proliferating cell   Cam5.2: Epithelial cell

0.25NAobjective

Resolution:2μm×2μm×5μm  



Hela cell

Treatedwith Nuohai cell expansion reagent kit

Mito:Mitochondrion

 Imaging by Confocal Microscope

Resolution:0.414μm×0.414μm×0.8μm    


U-2 OSCell

Treatedwith Nuohai cell expansion reagent kit

Tubulin:Tubulin

  Imaging by Confocal Microscope

Resolution:0.414μm×0.414μm×1μm  



Colorectal LymphNode

CK19:Epithelialcell   Lca: leukocyte surface antigen

 0.25NAobjective

Resolution:3.3μm×3.3μm×7 μm    



 Colorectalpolyps

Ki67: Proliferating cell   Cam5.2: Epithelial cell

Lca: leukocyte surface antigen

0.25NAobjective

Resolution:3.3μm×3.3μm×7μm    



Mouse brain-glioma tumor and nano material

Glioma(red),  nano material(green)


Tyrosine Hydroxylase staining reveals sympathetic nerve in an intact mouse heart

Cleared and labeled with our pipeline, imaged with LS18 at 3.2x.



小鼠心脏血管成像


Endogenous Thy1-driven EGFP expression visualized throughout a P15 mouse entire CNS (whole brain and spinal cord).

Cleared with our pipeline, imaged with LS18 at 4x



Thy1-GFP 阳性神经元


PI (Propidium Iodide) staining of an entire mouse brain hemisphere.

Cleared and labeled with our pipeline, imaged with LS18 at 3.2x.


Endogenous Tie2tdTomato visualized throughout an entire adult mouse brain.

Cleared with our pipeline, imaged with LS18 at 4x.


 

Endogenous Thy1-driven EGFP expression visualized throughout an entire adult mouse brain.

Cleared with our pipeline, imaged with LS18 at 3.2x


Neurons of brain sample in transgenic Thy1- EGFP mice.

Cleared with our pipeline, imaged with LS18 at 6.3x.


Tyrosine Hydroxylase staining reveals sympathetic nerve in mouse lung

Cleared and labeled with our pipeline, imaged with LS18 at 3.2X.



鼠肝脏神经


Endogenous Tie2tdTomato visualized in a mouse liver.

Cleared with our pipeline, imaged with LS18 at 3.2x.



Endogenous Tie2tdTomato visualized in a mouse stomach.

Cleared with our pipeline, imaged with LS18 at 3.2x.



Endogenous Tie2tdTomato visualized in a mouse kidney.

Cleared with our pipeline, imaged with LS18 at 3.2X.



Tyrosine Hydroxylase staining reveals sympathetic nerve in an intact mouse spleen

Cleared and labeled with our pipeline, imaged with LS18 at 6.3x.



Endogenous Tie2tdTomato visualized in a mouse testis.

Cleared with our pipeline, imaged with LS18 at 3.2x.



胎鼠卵巢

卵泡(红色)、细胞核(蓝色)


小鼠胚胎膨胀样品(细胞核&Lectin阳性细胞)

采用锘海组织膨胀试剂盒,LS18平铺光片显微镜6.3X倍率成像


小鼠胚胎神经



Mouse spleen labeled with Tyrosine Hydroxylase 

Cleared and labeled with our pipeline, imaged with LS18 at 23x


小鼠脊髓神经元


小鼠肠血管及细节图


小鼠骨股双通道(图左)  间充质干细胞(图中)  基质细胞(图右)


斑马鱼





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